Eppendorf 5402 | Manual

Eppendorf 5402 is a legacy refrigerated microcentrifuge known for its robust German engineering and reliable performance in temperature-sensitive laboratory work. Although it is an older model, often found as refurbished or for parts today, it remains a "workhorse" for routine molecular biology and biochemistry applications. SelectScience Core Specifications

Error 3: Usually indicates that no rotor is detected. Check the rotor installation.

• Read and understand the safety precautions.
• Ensure the rotor and accessories are properly installed and balanced.
• Select the appropriate centrifugation parameters (speed, time, temperature).

Introduction:

| Problem | Manual's Likely Cause | Solution | | :--- | :--- | :--- | | Centrifuge won't start | Lid not locked; motor thermal cutout | Check lid knob; wait 30 min for motor cooling | | Violent shaking at startup | Imbalanced load or loose rotor | Re-balance tubes; re-tighten rotor nut | | No deceleration brake | Brake resistor failed (electronics) | Service required (see repair manual) | | Unusual grinding noise | Motor bearing failure or debris in chamber | Stop immediately. Clean chamber. If noise persists, replace bearings |

Rotor Selection and Installation:

Operating the Centrifuge:

Why the Manual Still Matters

You might think, “It’s just a centrifuge. You put tubes in, close the lid, and press ‘Start.’” The 5402, however, is a creature of its time. It uses electromechanical buttons, a single-line LCD display, and—crucially—requires specific rotor-bucket-adapter combinations that are no longer intuitive. eppendorf 5402 manual

Maintenance and Troubleshooting